Journal: Scientific Reports

Article Title: Developmental timing of auditory deprivation influences spatial memory and hippocampal plasticity in rats

doi: 10.1038/s41598-025-26704-8

Figure Lengend Snippet: ( A ) Western blot analysis representative image bands of BDNF, synaptophysin, and PSD-95 in the hippocampus ( B ) Quantitative representation of the protein expression levels in the hippocampus, normalized to β-actin. Data are represented as mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001 show the comparison between control and CA groups and # P < 0.05, ## P < 0.01, and ### P < 0.001 show the comparisons between the groups. No splicing or image manipulation was performed. The PVDF membranes were not cut before antibody incubation. Only grayscale conversion and uniform brightness and contrast adjustments were applied to improve clarity. Full-length blots are available in Fig. S2.

Article Snippet: The membranes were blocked using bovine serum albumin (BSA) in 0.1% Tween 20 for 1 h. Then, the membranes were incubated overnight at 4oC with specific primary antibodies: rabbit anti- BDNF antibody (orb10181, Biorbyt, Wuhan, China, 1:300), rabbit Anti-PSD95 antibody (orb500730, Biorbyt, Wuhan, China, 1:1000), rabbit anti-Synaptophysin antibody (orb704356, Biorbyt, Wuhan, China, 1:600), and rabbit anti-beta Actin antibody (GTX109639, Genetex, USA, 1:1000).

Techniques: Western Blot, Expressing, Comparison, Control, Incubation